Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine

نویسندگان

  • A.A. Aslaminejad Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • M. Doosti Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • M. Pasandideh Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
  • M. Raouf Delgosha Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • M. Tahmoorespur Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • M.R. Nassiri Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • R. Pasandideh Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • S. Zibaei Razi vaccine and Serum Research Institute, Mashhad, Iran
چکیده مقاله:

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes wereupregulated. The expression of TNF-α and IL-10 genes significantly increased (P

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منابع مشابه

evaluation of cytokine mrna expression in vaccinated guinea pigs with foot-and-mouth disease type o inactivated vaccine

foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyz...

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عنوان ژورنال

دوره 71  شماره 1

صفحات  15- 19

تاریخ انتشار 2016-03-01

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